Genetics, disease activity influences the microbiota in spondyloarthritis patients

September 07 2022

2 minutes to read

Disclosures:
The authors reported receiving a support grant from the French National Research Agency, Help Publique – Hôpitaux de Paris and La Fondation Arthritis R&D.


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In patients with spondylitis, genetic factors and levels of disease activity likely influence gut microbiota composition, according to data published in arthritis & rheumatism;

“This multifaceted disease is thought to result from complex interactions between genetic background and environmental factors,” Magali Berland, Ph.D., from Paris-Saclay University in France, co-authors wrote. “Looking at it so disorganized The microbial composition of the guti.e. dysbiosis, is the hallmark of [inflammatory bowel disease (IBD)], the previous observations support the hypothesis that bacterial dysplasia could also contribute to the pathogenesis of SpA. “

Consultation of the doctor and the female

In patients with SpA, genetic factors and disease activity levels likely influence gut microbiota composition, according to the data. Source: Adobe Stock

To further investigate the role of germs and genetics in SpA . patientsBerland and colleagues collected stool samples from 197 volunteers. Patients with SpA, all of whom were enrolled through a tertiary care center, were required to fulfill the International Society Classification Guidelines for the Evaluation of Spondylitis Spondylitis and to abstain from antibiotic use for at least 1 month prior to sample collection. In addition, patients cannot undergo preparatory procedures for colonoscopy for at least 6 months. The researchers also enrolled 40 volunteers who were siblings of patients covered with SpA.

The authors used shotgun sequencing on DNA isolated from stool samples. Metagenomic Species Pangenome (MSP) was used to identify and quantify the presence of specific microbes. Finally, the potential of the microbiome was assessed through an internal pipeline. The authors used species abundance tables to perform intestinal profiling and their corresponding intestinal phenotypes Bacteroides, Firmicutes, and PrevotelAnd the The authors wrote. In all, the analysis included samples from 102 patients with SpA and 63 healthy controls.

According to the researchers, dysbiosis was confirmed more in patients with SpA than in healthy controls. In addition, intestinal phenotypes of bacteria were more common in patients with severe SpA, compared to healthy controls. The researchers also found that diversity restriction was more often present in patients who showed severe disease activity. Bacterial prevalence was also associated with an indicator of ankylosing spondylitis disease activity in the bathroom. galaxy Navos It was one of the most widely recognized and distinguished species.

In patients with and without SpA, there was no significant difference in MSP richness between patients with SpA and healthy controls, according to the researchers.

“Or not, The extent of dysbacteriosis Berland and colleagues write that it appears surprisingly related to disease activity. Second, the HLA-B27 genotype, which strongly predisposes to SpA, was associated with significant dyspareunia in the patients’ healthy siblings, so inclusion of this group of [health controls (HC)] In the full primary analysis resulted in fairly minor differences between SpA and HC.

“Analysis of potential interceptors – especially drugs – is always an interesting point because it may influence the composition of the gut microbiota,” they added. “In our analysis, we did not observe a strong effect of the drug on dysbiosis, but that does not mean that the drug the patients are taking has no effect at all.”

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